Volume : 4
Issue : 1
Online ISSN : 2394-2126
Print ISSN : 2394-2118
Article First Page : 30
Article End Page : 33
Background: The aim of the study was to examine relationship between severity of stress and onset/completion of erythrocyte haemolysis in normal healthy individuals.
Materials and Methods: The study was conducted in Physiology department, Deccan College of Medical Sciences, Hyderabad, after approval from the Institutional Review Board. The study included 50 healthy individuals of both the sexes aged between 20 to 50 years. Individuals working for minimum 8 to 10 hours a day were included and individuals suffering from hypertension, obesity, diabetes mellitus, musculo-skeletal disorders, sickle cell disease and goitre were excluded. Osmotic fragility was determined by collecting 2ml of blood from each individual and stored in EDTA tubes. A drop of blood was added to saline solutions of different tonicity arranged in 12 test tubes. The test tubes were seen for onset and end of hemolysis after an hour.
Stress was measured by Cohen’s perceived stress questionnaire and individual were divided into 3 groups viz. low, moderate and high stress categories as per their scores.
Results: Findings were analysed by student’s t-test and completion of hemolysis was earlier in severe stress group compared to mild and moderate. There is no significant difference between males and females regarding point of onset of hemolysis (p=0.41) and end of hemolysis (p=0.37). Stress increases fragility and it was found that RBC fragility was significantly related to severity of stress. Fragility is not affected by gender.
Conclusion: RBC fragility was significantly related to severity of stress and it was found that fragility is not affected by gender.
Keywords: Osmotic Fragility, Erythrocyte, Hemolysis, Stress, Cohen’s Stress Scale