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Indian Journal of Clinical Anatomy and Physiology


Study of invitro antimicrobial activity of biogenically synthesised silver nanoparticle using leaf extract of aponogeton natans


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Author Details: Amiya Kumar Prusty, Saroja Kumar Patro

Volume : 2

Issue : 2

Online ISSN : 2394-2126

Print ISSN : 2394-2118

Article First Page : 60

Article End Page : 64


Abstract

Different biological methods are gaining recognition for the synthesis of silver nanoparticles (Ag-NPs) due to their multiple applications. One of the most important applications of Ag-NPs is their use as an antibacterial agent. The use of plants in the synthesis of nanoparticles emerges as a cost effective and eco-friendly approach. In this study preparation of silver nanoparticles by using leave extract of Aponogeton natans has been investigated. Characterization of different properties of the prepared nanoparticles by techniques like, UV-VIS spectroscopy, Zetasizer, SEM, and FT-IR was carried out. In-vitro antibacterial study of the prepared nanoparticles on different microorganisms like Staphylococcus aureus, Bacillus subtilis, Escherichia hermanii, and Pseudomonas aeruginosa was carried out. The results showed that silver nanoparticles in the range of 45-80 nm in size were synthesised. The silver nanoparticles showed the antimicrobial activity against Gram positive and Gram negative bacteria. Aponogeton natans was found to display strong potential for the synthesis of silver nanoparticles that can be used as antimicrobial agents by rapid reduction of silver ions.

Keywords:
Silver, Aponogeton natans, nanoparticles, Antibacterial