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Indian Journal of Clinical Anatomy and Physiology


A study of the histological and morphometric changes in jejunum of albino rats after administration of Indomethacin


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Author Details: Vinay Kumar, Manisha Gupta

Volume : 4

Issue : 3

Online ISSN : 2394-2126

Print ISSN : 2394-2118

Article First Page : 377

Article End Page : 380


Abstract

Indomethacin is developed as anti-inflammatory agent and introduced for the treatment of arthritis mainly rheumatoid arthritis in 1960s.The gastrointestinal tract is main target of NSAIDs toxicity. Indomethacin is the commonest of NSAIDs used by people. World over 35 million people consume this drugs daily. Indomethacin is a synthetic non-steroidal anti –inflammatory drug. The Indomethacin decreased the production of prostaglandins that sensitizes nociceptors to inflammatory mediators such as bradykinin and 5-hydroxytryptamin. This study was done in the Department of Anatomy, Himalayan Institute of Medical Sciences, Swami Ram Nagar, Dehradun over a period of 1 year. Albino rats of strain Charles Foster 16 (08males and 08 females) weighing 120gms (+10gms) obtained from the Central Animal House, HIMS, Dehradun (Uttarakhand) were used as experimental animals after obtaining the approval of IAEC. The rats were randomly divided into 4 groups, Group I, II and III and IV for the study. Each group was comprised of 04 rats out of which group I was taken as control and the remaining three groups were taken as the experimental groups. Each experimental group was subjected to oral administration of Indomethacin for 1 week, 2 weeks & 3 weeks respectively. The experimental and control groups were sacrificed at the end of 1st, 2nd and 3rd week intervals after giving ether anesthesia. They were immediately fixed on a wooden block with the help of paper pins. Dissection was done to open the abdomen for gross observations. Jejunum preserved in neutral buffered formalin. Staining was done with Harris’ Hematoxylin and Eosin. 3-5 µm thick paraffin sections were studied under light microscope and the histological changes was studied. The histological changes seen as decrease in the height of villi, Increased in cells -goblets, columnar, mitotic number per crypt were observed.

Keywords:
GIT, NSAIDS, ALBINORATS, Hematoxylin and Eosin Institutional Animal Ethical Committee (IAEC)

Doi No:-10.18231