International Journal of Clinical Biochemistry and Research

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Current Understanding in the Measurement of Low Density Lipoprotein Cholesterol: A Comparison of Direct Measurement by Homogenous Assays versus Calculations

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Author Details: Neha, Jaskiran Kaur, Anju Sharma, Ridhum, Amandeep Kaur

Volume : 3

Issue : 2

Online ISSN : 2394-6377

Print ISSN : 2394-6369

Article First Page : 162

Article End Page : 168

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Abstract

Background: To compare the results obtained by direct homogenous assay for LDL-C to those obtained by Friedwalds and Anandrajas formulas with the assumption that the results obtained by direct assay are most accurate. This was a comparative study for the estimation of LDL-C using two different types of calculation formulas and direct estimation of LDL-C by homogenous method.
Method: Serum Lipids and lipoproteins were measured in 505 fasting samples.  Serum Total cholesterol was measured using CHOD-PAP method first described by Stadman on Siemens Dimensions Clinical Chemistry RXL Analyzer. Triglycerides were measured by Glycerol Phosphate peroxidase - PAP method. Direct LDL-C was measured by a homogenous assay by siemens diagnostics. A- HDL was measured by a homogenous method which uses PEG- cholesterol esterase using kit from siemens diagnostics. This was a comparative study for the estimation of LDL-C using two different types of calculation formulas and direct estimation of LDL-C by homogenous method.
Results: A good correlation was found between D-LDL as compared to both F-LDL and A-LDL. Pearsons coefficient of correlation between F-LDL & D-LDL was 0.891 (p<0.001) which was comparatively better than that between A-LDL & D-LDL which came out to be 0.850.
Conclusion: In conclusion, regarding patients convenience, financial reasons and accuracy we support the reliability of Anandrajas formula as indirect low density lipoprotein estimation – in Punjabi population.   

Keywords:  Anandraja’s LDL, Friedwald’s LDL, Beta Quantification, Calculated LDL, Direct LDL-C