International Journal of Clinical Biochemistry and Research


Comparison of the analytical techniques of hba1c estimation by immunoturbidimetric and HPLC methods in diabetic and pre- diabetics patients


Full Text PDF Share on Facebook Share on Twitter


Author Details: A .G. Thivyah Prabha

Volume : 4

Issue : 2

Online ISSN : 2394-6377

Print ISSN : 2394-6369

Article First Page : 187

Article End Page : 190


Abstract

Aims & Objectives: The aim of the study is to compare the available analytical methods of measurement of HbA1c like immunoturbidimetric and HPLC methods.
Materials and Method: Total of 150 pre-diabetic and diabetic patients are included in the study. HbA1c was measured by immunoturbidimetric and HPLC methods as these are the common methods used by the clinical laboratories.
Results: The HbA1c values of two methods have a significant difference. The value of immunoturbidimetric method was less than the HPLC method. The two methods correlated with the correlation coefficient of 0.972.
Conclusion: Both the methods are reliable and show a strong correlation .Even though the immunoturtbidimetric values are low it is specific and more cost effective than the HPLC methods. HPLC is a sensitive method.

Keywords:
HbA1C, Immunoturbidimetric, HPLC, Diabetics, Prediabetics