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IP Indian Journal of Conservative and Endodontics


In vitro antimicrobial efficacy of photoactivated cow urine against enterococcus faecalis


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Author Details: Anitha Rao S., Sunitha Lakkireddy, Nageshwar Rao B., Venkateshwar Rao G.

Volume : 1

Issue : 1

Online ISSN : 2581-8988

Print ISSN : 2581-9534

Article First Page : 13

Article End Page : 16


Abstract

Introduction: The success of the endodontic treatment depends on the complete debridement of the root canal to prevent infection. E. faecalis is the predominant bacteria responsible for endodontic treatment failure. Various irrigants are used to disinfect the root canals and eradicate the microorganisms. Sodium hypochlorite (NaOCl) is the irrigant of choice in endodontic practice. However, there is a need for alternative irrigant to overcome the shortcomings caused by the use of sodium hypochlorite.
Materials and Methods: A total of 75 uniradicular teeth without fractures and caries were collected, decoronated and irrigated with 2.5% NaOCl to remove any remnants. Canals were prepared using protaper universal rotary files upto F3 size and the teeth were inoculated with E.faecalis. The contaminated teeth were randomly divided into group 1 (n=15) Control, group 2 (n=15) 2.5% NaOCl (Positive control), Group 3 (n=15) photo-activated cow urine and incubated for 60, 90 and 120 min at 37oc. Colony forming units and percentage reduction was determined at various tested time intervals. The obtained data is statistically analyzed using student t test.
Results: Both the groups treated with 2.5% sodium hypochlorite and photoactivated cow urine, exhibited effective inhibitory potential E.faecalis when compared to control group. The mean number of colonies formed was 0 and the percentage reduction was 100% in 2.5% NaOCl group. The photoactivated cow urine group exhibited inhibitory potential with increase in exposure time showing 85% reduction at 120 min.
Conclusion: From the study, it can be concluded that photoactivated cow urine can be used as an alternate irrigant to eradicate the E.faecalis bacteria from the root canal systems.

Keyword: 
Antibacterial Activity, Enterococcus faecalis, Photoactivated cow urine, Sodium hypochlorite