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IP Indian Journal of Conservative and Endodontics


Comparative evaluation of the efficacy of laser and plasma microjet on root canals infected with enterococcus faecalis - An in vitro-study


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Author Details: Shikha Gandhi, Ashwini Gaikwad

Volume : 2

Issue : 2

Online ISSN : 2456-8953

Print ISSN :

Article First Page : 55

Article End Page : 60


Abstract

Introduction: Aim of this in vitro study was to compare the anti-microbial efficacy of diode laser and plasma microjet with sodium hypochlorite in root canals infected with E. faecalis.
Materials and Method: Teeth were sterilized after decoronation and preparation of the root canals. A liquid culture suspension of 0.5 McFarland standard BHI broth was prepared to obtain 1.5× 10⁸ colony forming units/ml from a subculture of E.faecalis. Sterilized teeth were incubated with the bacterial suspension for a week under aerobic and static conditions at 37°C. Based on their treatment, the samples were divided into 2.5% sodium hypochlorite (control group, n=15), diode laser (n=15) and plasma microjet (n=15). Sterile paper points were introduced to working length, allowed to saturate, and were transferred to tubes containing 1 ml of freshly prepared BHI broth and vortexed for 20 seconds. After 10 fold serial dilutions, aliquots of 0.1 ml were plated onto blood agar plates and incubated. The colony forming units grown were counted and recorded.
Result: There was a statistically significant difference observed among the 3 groups using ANOVA test. Further Tukey’s post hoc analysis showed significant difference between groups A & C, and groups B & C. No difference was found between groups A & B.
Conclusion: Diode laser and sodium hypochlorite proved to be equally efficacious in their anti-microbial properties against E. faecalis. Plasma microjet exhibited least antimicrobial activity as compared with diode laser and sodium hypochlorite.

Keywords:
Sodium hypochlorite, Diode laser, Plasma, Anti-microbial efficacy, E. faecalis

Doi No:-10.18231