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Indian Journal of Clinical and Experimental Ophthalmology

Microbiological regional profile of infective keratitis

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Author Details: Reena Gupta, Chekitaan Singh, Bella Mahajan, AK Khurana

Volume : 1

Issue : 4

Online ISSN : 2395-1451

Print ISSN : 2395-1443

Article First Page : 264

Article End Page : 272


Purpose: To determine epidemiological characteristics, specific pathogenic organisms, risk factors for infective keratitis.
Methods: Patients with signs and symptoms of infective keratitis were included. History, examination including visual acuity, slit-lamp bio-microscopy, microbiological examination including Gram staining, KOH mount, culture, sensitivity of corneal scrapings were done.
Results: Forty cases were evaluated, thirty (75%) were males, ten (25%) were females. Age ranged from 5 - 70 years, 35% in age group up to 30 years, 50% between 31-60 years, 15% were > 60 years of age. Right eye was involved in 55%, left in 45%. Highest incidence of ulcers occurred in farmers and labourers (65%). History of trauma was present in 80% patients, ocular surface disease in 7.5%. Diabetes was present in 12.5%. 57.5% patients had hypopyon on presentation. Based on culture reports 62.5% had bacterial keratitis, 25% fungal keratitis and 12.5% sterile. 80% bacterial isolates were gram positive and 20% gram negative. Staphylococcus aureus(40%) was the commonest organism cultured followed by Staphylococcus epidermidis(32%), Pseudomonas(12%), Pneumococcus(8%) and Acinetobacter(8%). Aspergillus was isolated in 60% of cases and Fusarium in 40%. Gram positive isolates were maximally sensitive to Cefazoline, gram negative isolates to Gentamicin.
Conclusion: Males in rural agricultural population in economically productive age group are most vulnerable to infective keratitis. Culture on blood and chocolate agar detects more number of organisms compared to Gram stain alone. Staphylococcus aureus was the most common bacterium and Aspergillus most common fungus isolated. Cefazoline and Gentamicin combined cover most bacterial isolates.

Key Words:
Infective Keratitis2, epidemiological profile 1, risk factors3