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IP International Journal of Medical Microbiology and Tropical Diseases


The Role of Elyc Protein in Protection Against Oxidative Stress in E. Coli Cells


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Author Details: Khadidja Senouci-Rezkallah

Volume : 1

Issue : 1

Online ISSN : 2581-4761

Print ISSN : 2581-4753

Article First Page : 13

Article End Page : 23


Abstract

Bacteria are covered by a complex envelope that delimits the cell and protects it against variations in osmotic pressure and environmental stresses. The goal of our study was the characterization of the importance role of Conserved inner membrane protein ycbC (elyC) gene in cell wall assembly and oxidative stress response in Escherichia coli. E. coli WT cells, ΔelyC and ΔmrcB mutants were grown in LB medium 37°C and 22°C. After that, the hydroxyl radical level was measured by the Flow cytometry (FACS). RNA extraction and purification was achieved and transcriptional analysis by RT-PCR was performed. Our results demonstrate the increase of Hydroxyl radical (oxidative stress) in ΔelyC mutant compared to WT and ΔmrcB mutant. Our data show the up-regulation of genes encoding for ycbC gene, the genes encoded for the enzymes implicated in PG synthesis and oxidative stress response in WT cells grown at 22°C. Furthermore, ΔelyC and ΔmrcB mutants grown at 37°C and 22°C revealed the overexpression of genes encoding enzymes involved in PG synthesis compared to WT cells. Moreover, gene expression of genes encoded for enzymes implicated in oxidative stress response in ΔelyC mutant was over-expressed but not changed in ΔmrcB mutant. These results approve the significant role of ElyC protein in Gram-negative bacterial envelope assembly and report a possible connection between cell wall biogenesis by ElyC factor and oxidative stress defense in Escherichia coli.

Keywords
: Envelope biogenesis, Enterobacterial Common Antigen (ECA), peptidoglycan (PG) synthesis, ElyC (YcbC) factor, flow cytometry (FACS)