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Indian Journal of Microbiology Research

A Study on Bacteriological Profile and Antimicrobial Susceptibility Pattern of Sputum Samples in Patients with Lower Respiratory Tract Infections a Tertiary Care Hospital

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Author Details: Nithya Chinnnusamy, Dillirani Vedachalam, Vijayalaksmi Arumugam

Volume : 3

Issue : 1

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 27

Article End Page : 30


Background & Objectives: The morbidity and mortality still remain significantly high in patients with severe lower respiratory tract infections. But data related to the offending organisms and their antibiotic sensitivity pattern is limited. Therefore, this study was designed to identify the bacterial pathogens and their antibiotic sensitivity of sputum samples in patients with severe lower respiratory tract infections which were received by Microbiology Department, between January 2015 to December 2015.
Methods: The present study was conducted during a one year period (January to December 2015). A total of 130 sputum samples were processed. All the samples were processed through Gram’s stain and culture. The culture was identified by standard protocols and antibiotic susceptibility was performed by Kirby Bauer disc diffusion method.
Results and conclusion: Out of the 130 sputum culture samples, significant pathogens were isolated in 82 [63%] sputum samples. Among the isolated organisms, Klebsiella pneumoniae [31.7%] was the commonest isolated organism, followed by pseudomonas spp [14.63%]. Majority of isolates were only sensitive amikacin, Pipracillin- tazobactum and imipenem. Staphylococcus aureus was isolated in 11 samples and 6 were Methicillin resistant Staphylococcus aureus [MRSA] which were sensitive to Vancomycin. There were a significantly high proportion of patients who were affected by drug resistant strains. Before starting empirical antibiotics on patients with moderate to severe lower respiratory tract infections, it is always advisable to obtain sputum for culture and antibiotic sensitivity test.

Klebsiella pneumoniae, Pseudomonas sp, Sputum, MRSA, Sputum Culture