Indian Journal of Microbiology Research

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A study on extended spectrum β lactamase & metallo β lactamase producing bacteria from drinking water sources in and around Kelambakkam

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Author Details: Danish Vijay Devaraj, Jayanthi Sivasubramaniyan, Shameem Banu AS

Volume : 3

Issue : 3

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 255

Article End Page : 261

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Abstract

Introduction: Water is essential to life and many people do not have access to clean safe drinking water. Poor sanitation is the major cause for water contamination. Death due to infection like cholera, typhoid fever, bacillary dysentery are transmitted through contaminated water.
Aims & Objectives: To study the bacteriological profile of drinking water samples, to perform antimicrobial sensitivity testing of the isolates and genotypic identification of resistant gene.
Materials and Methodology: Drinking water sample were collected from in and around Kelambakkam for a period of 1year. Antimicrobial susceptibility testing was carried out for the isolates according to standard guidelines. ESBL producers were detected using double disc synergy test method. MBL producer were detected by combined disk test. Screening for bla _TEM, bla _VIM gene among the drug resistance isolates were done.
Result: Among 161 samples from various sources, most common isolates were Escherichia coli (n=41) 25.4%, followed by Klebsiella spp. Out of 41 isolates of E.coli were 23 and 10 were ESBL and MBL producer respectively. Among 10 isolates 8 were both ESBL and MBL producer,  isolates positive for bla _TEM gene & bla _VIM gene were 5 & 2 respectively.

Keywords: E.coli, Drinking water, ESBL and MBL