Indian Journal of Microbiology Research


Tube adherence test as a screening tool for detection of biofilm formation among Staphylococcus


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Author Details: Sathish J.V., Ram Mohan Reddy K., Sujith Pavan

Volume : 4

Issue : 1

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 44

Article End Page : 47


Abstract

Background: Due to large number of infections caused by biofilm producing organism, a reliable and reproducible method for its detection is necessary. Specific methods like Polymerase Chain Reaction (PCR) are available for detection of biofilm, but these are beyond the scope majority of laboratories in developing nations like India.
Objective: To test reliability of Tube Adherence test as a screening tool for detection of biofilm formation among Staphylococcus.
Materials and Methods: Various clinical samples were collected with universal precautions from patients with indwelling medical device for more than 48 hours. Screening for biofilm detection was done using Tube adherence method.
Results: Tube method was 97.5% sensitive and 100% specific for detection of biofilm formation.
Conclusion: Tube method can be used as a general screening method for detection of biofilm formation by Staphylococci.

Keywords:
Biofilm, Predictive Value, Sensitivity, Specificity, Staphylococci, Tube Method