PRODUCTION AND PARTIAL PURIFICATION OF ALKALINE PROTEASE FROM BACILLUS CIRCULANS MTCC 7906 USING POTATO PEEL AS SUBSTRATE
Background: The present study was carried out to use potato peel which is available in plenty from food processing industries as a substrate for alkaline protease production using B. circulans MTCC 7906. Method: The potential of potato peel as substrate for alkaline protease has been studied for its production and purification was studied by Bacillus circulansMTCC 7906. In order to make the process economically viable the casein was replaced by Potato peel. The results were analyzed using factorial CRD.
Results: Alkaline protease activity was increased by2.74fold and was obtained in 96 h of fermentation. Ammonium sulphate precipitation followed dialysis of the fraction 60-90% saturation with maximum specific activity was partially purified using DEAE column chromatography. Purification profile was studied and the fraction with highest specific and enzyme activity were pooled to achieve specific activity of 18.761 U/mg with 6.185 fold purification. The concentration of enzyme was done using PEG had the specific activity of 69.332 U/mg and 22.859 fold purification.
Conclusion: The concentrated partially purified enzyme was subjected to SDS PAGE followed by silver staining for both the substrates casein and potato peel showed the protein band for alkaline protease at 40 kDa revealing the purity of the enzyme. Experiments revealed successful utilization of potato peel as an economical substrate for enhanced production of alkaline protease by B. circulans MTCC 7906. Projects which are inclined to reduce global wastage should be encouraged and this is one of the same type.
Key words: Alkaline protease, Bacillus circulans, potato peel, purification, production.