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Indian Journal of Microbiology Research


EFFICACY OF DRIED BLOOD SPOT IN EARLY INFANT DIAGNOSIS OF HIV INFECTION


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Author Details: Asima Banu, Eswari. L, Zahid Fazal. A

Volume : 2

Issue : 1

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 27

Article End Page : 29


Abstract

Introduction: Nucleic acid analysis has been the most reliable diagnostic technique for detecting HIV infection in the neonates because of the presence of maternal antibodies in infants less than 6 months. As this is an expensive test, Direct Blood spot was used to detect HIV infection in early infanthood to prove the technical and economical advantage over the whole blood assays.
Methodology: 115 infants of HIV seropositive mothers were subjected to DBS test and subsequently to Antibody and whole blood PCR analysis. The results of the DBS sample titres were compared with the standard whole blood analysis reports.
Results: Of the 115 infants who underwent the diagnostic tests, 10 children were found to be HIV positive of which 50%were found positive with the HIV antibody tests and the rest were DBS positive. The sensitivity and specificity of DBS was found to be 100% compared to the whole blood PCR analysis.

Conclusion: DBS is an ideal choice as a screening tool for detecting HIV infection in early infant hood.