RESISTANCE PATTERN OF PSEUDOMONAS AERUGINOSA ISOLATES FROM SURGICAL WOUNDS
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Background: Psudomonas aeruginosa is a major cause of nosocomial infections. Despite advances in sanitation facilities and the introduction of wide variety of antimicrobial agents with antipseudomonal activities, life threatening infections caused by this agent continue to cause devastations in the hospitals.
Aims & objective: To study the Resistance Pattern of Pseudomonas Aeruginosa Isolates From surgical wounds.
Material & Method: This study was a retrospective study done in Department of Microbiology, Index Medical College hospital and research center, Indore, m.p, india from March 2014 to december 2014. A total of 300 isolates of Pseudomonas aeruginosa both from indoor patients and patients attending outpatient department who were having surgical wound infections, were included in the study. Each isolate was evaluated for susceptibility to nine different antibiotics i.e cefotaxime, ceftriaxone, ceftazidime, cefdinir, amikacin, gentamicin, ciprofloxacin, piperacillin and imipenem. ESBL production was detected by double disc potentiation method by applying disc of cefoperazone [75μ] and combination of cefoperazone-sulbactam [75/30μ]. The results were interpreted according to Clinical Laboratory Standard Institute (CLSI) guidelines.
Result: Maximum resistance was seen to third generation cephalosporins-68.5 % to cefotaxime, 80.23% to ceftriaxone, 75.5% to ceftazidime, 93% to cefdinir. Amikacin showed resistance in 42.5% and Gentamicin in 81% of the isolates. Ciprofloxacin resistance was seen in 72.5% isolates while piperacillin resistance was seen in 43% of the isolates. Minimum resistance was seen to imipenem -2.5%
Conclusion: The present study highlights that the Pseudomonas aeruginosa remains an important cause of nosocomial wound infections. Is study thus gives the alarming signal for the future, making the therapeutic options more difficult. Strict infection control measures are to be followed to contain the so called water and soil organism as Pseudomonas aeruginosa.