Indian Journal of Microbiology Research

DNA fingerprinting and drug resistance profiles of Mycobacterium tuberculosis strains isolated from HIV-infected pulmonary tuberculosis patients

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Author Details: Santosh S. Patil, Mohite Shivajirao T, Subarna Roy, Shuchismita Dey

Volume : 4

Issue : 2

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 210

Article End Page : 215


Introduction: Tuberculosis (TB) is a leading cause of morbidity and mortality among HIV/AIDS patients. These patients are more likely to develop resistance to anti-tuberculosis drugs as well. Identifying open cases of TB, studying their drug susceptibility pattern and knowledge of genetic diversity to know transmission dynamics among HIV-TB patients is therefore important to control TB.
Aims: 1) To determine the prevalence of HIV, among sputum positive pulmonary tuberculosis (PTB) patients. 2) To study drug resistance pattern of Mycobacterium tuberculosis isolates. 3) To evaluate genotypic diversity of M. tuberculosis isolates using random amplified polymorphic DNA (RAPD) technique.
Settings and Design: Hospital based cross sectional study
Materials and Method: M. tuberculosis strains were isolated from HIV seropositive PTB patients with irrespective of their anti-TB treatment. These isolates were processed for drug sensitivity testing (DST) by Proportion method and subsequently genotyped by RAPD technique.
Results: Prevalence of HIV-TB co-infection was 22.2%. Total 52.1% M. tuberculosis isolates were resistant to one or more number of drugs. We report 17.4% MDR-TB among study population. RAPD-PCR showed excellent discrimination with high degree of polymorphism among M. tuberculosis isolates. Transmission and diversity of tuberculosis was 10% and 90% respectively.
Conclusions: 17.4% of MDR-TB among studied patients indicated worrisome trend that calls for continuous monitoring and DST. RAPD analysis is quick, simple and has potential for molecular epidemiology of TB. RAPD-PCR showed notable genetic polymorphism, suggesting that source and type of infections within the population were varied and no any particular clone of M. tuberculosis is spreading among study population.

HIV-TB, RAPD-PCR, Pulmonary tuberculosis, DNA fingerprinting