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Indian Journal of Microbiology Research


Prevalence of extended-spectrum β-lactamases producing escherichia coli in urinary specimens and their phenotypic detection by modified three-dimensional enzyme extract test: Comparison with the Phen


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Author Details: Arvind Khodare, Anita Mutha, Manish Purohit

Volume : 4

Issue : 3

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 244

Article End Page : 247


Abstract

Escherichia coli is the most common cause of UTI in community as well as in hospitalised patients. Detection of ESBLs producing organisms from samples such as urine may be important because this represents an epidemiologic marker of colonization, and therefore there is potential for transfer of such organisms to others.
Aims: The purpose of this study was to know prevalence of extended spectrum β-lactamases (ESBLs) in strains of Escherichia coli isolated from urine specimens and comparison of PCDDT and IMTDT of ESBL detection.
Settings and Design: Department of Microbiology, MGM Medical College, Indore. Cross sectional study.
Materials and Method: A total of 200 Escherichia coli strains isolated from 1500 consecutive non repeating urine specimens selected for the study. They studied for ESBL production phenotypic confirmatory disc diffusion test (PCDDT) and indirect modified three-dimensional enzyme extract test (IMTDT).
Statistical analysis used: Kappa statistics.
Results: 86% (171) of the isolates found to be resistant to atleast one of the 3rd generation cephalosporines tested (cefpodoxime, cefotaxime, ceftazidime and ceftriaxone). ESBL detected in 99 (58%) of the Escherichia coli by PCDDT and in 76% (130) by IMTDT.
Conclusions: Our study shows prevalence of ESBL producing Escherichia coli in urinary isolates. IMTDT found to be better than PCDDT for the detection of ESBL. The overall prevalence of ESBL producing uropathogenic Escherichia coli found to be 49.5% for our institute.

Keywords:
ESBL, Three dimensional test, Uropathogenic E. coli, UTI