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Indian Journal of Microbiology Research


Comparison between ELISA and chemiluminescence immunoassay for the detection of Hepatitis C virus antibody


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Author Details: Pampi Majumder, Anup Kumar Shetty

Volume : 4

Issue : 4

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 353

Article End Page : 357


Abstract

Detection of antibody to Hepatitis C Virus (Anti-HCV) by Enzyme linked immunosorbent assay (ELISA) method is one of the most popular method and today it's being slowly replaced by more sensitive and rapid automated analyzer chemiluminescence immunoassay (CLIA). This was an observational cross-sectional study and aim of this study was to compare ELISA and chemiluminescence immunoassay (CLIA) for the detection of anti-HCV. A total of 91 samples were tested for the detection of anti-HCV by CLIA and ELISA. Out of 91 samples, 30 (33%) were non-reactive by both CLIA and ELISA. 32(35.2%) samples were reactive by CLIA, 2(6.25%) CLIA reactive samples were found to be non-reactive by ELISA. 29 (31.87%) samples were interpreted as borderline on CLIA. By using ELISA technique as gold standard for Anti-HCV detection our results showed 96.07% specificity and 96.66% sensitivity for CLIA technique. Detection of anti-HCV by CLIA is comparable with ELISA. CLIA can help in detecting early infection compared to ELISA and is suitable in large sample volume laboratories.

Keywords:
ELISA, CLIA, Anti-HCV antibody, Comparison