Expression of Assimilatory Nitrate and Nitrite Reductase of Rhizobium meliloti
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Anup Kumar Halder, P. K. Chakrabartty
Volume : 2
Issue : 3
Online ISSN : 2394-5478
Print ISSN : 2394-546X
Article First Page : 133
Article End Page : 137
Purpose: To investigate the expression of assimilatory nitrate- and nitrite reductase activity of Rhizobium meliloti SU 47.
Methods: Rhizobium cell were grown in medium, after 20 hrs cells were washes with potassium phosphate buffer and assayed the enzyme activity through nitrite estimation.
Result: Expression of assimilatory nitrate- and nitrite reductase was observed in GTS/glutamate medium. The enzymes were inducible in nature since it expressed only in presence of nitrate or nitrite. Ammonium inhibited the enzyme activity. Cyanate acted as a gratituous inducer of both the enzymes. Molybdenum acts as modulator whereas tungstate as an inhibitor of the enzyme.
Conclusion: Both the enzymes, assimilatory nitrate- and nitrite reductases expressed in GTS/glutamate medium in presence of nitrate.
Key Words: Assimilatory nitrate and nitrite reductase, Rhizobium meliloti, Cyanate