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Indian Journal of Microbiology Research


Etiological Study of Neonatal Septicemia


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Author Details: Pragati Abhimanyu Bulle, Ashwini Patil

Volume : 2

Issue : 3

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 142

Article End Page : 147


Abstract

Introduction: Neonatal sepsis is a clinical syndrome of bacteremia characterized by systemic signs and symptoms of infection in the first month of life

Aims and objective
1. To study the etiological profile of neonatal septicemia.
2. To determine the antibiotic sensitivity pattern of the bacterial isolates.
Material and Method: The study was undertaken for a period of 2 years in the diagnostic microbiology laboratory GMC Nagpur. A total 228 clinically suspected neonatal septicemia cases were studied and blood cultures were performed with help of BaCT-ALERT 3D system, isolates were identified with standard biochemical test and subjected for antimicrobial susceptibility test. CRP test of all suspected cases were also done.
Results: There were 139 (60.96%) male cases and 89 (39.04%) female cases with M: F ratio of 1.56:1. The 69.29% were of early onset septicemia and 30.71% were of late onset septicemia. The 108 (47.36%) blood cultures show growth out of which 35 (32.41%) gram positive , 70(64.81%) gram negative and 3 (2.78%) were candida albicans. time to positivity of blood culture 25% were positive within 24 hours, 82.41% by 1-2 days, 94.44% by 2-3 days and 99.07% by 3-4 days. Klebsiella pneumoniae (28.57%) were commonest organism in gram negative and Staphylococcus epidermidis (CONS) (45.71 %) were commonest organism in gram positive. The 25% of gram negative isolates as ESBL producers. 70% isolates of S. aureus were MRSA. No MBL and no VRSA detected. CRP test were positive (CRP ≥ 0.6 mg/dl) in 152 (66.67%) cases with a sensitivity of 69.08% and specificity of 96.05%. Overall mortality rate was 23.18%. Maternal fever and low birth weight were major risk factors.
Conclusion: Neonatal septicemia is a life threatening emergency. The study of etiological profile and their antibiotic sensitivity pattern plays a significant role.
 
Key words: Neonatal, Septicemia, Blood, Culture, BACT/ALERT