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Indian Journal of Microbiology Research


Characterization, Antibiotic Resistance Pattern and Detection of Metalloβ-lactamases and Amp C in Pseudomonas aeruginosa in a Tertiary Care Hospital


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Author Details: Amit Rajshekar Ugargol, Shilpa Karnum

Volume : 2

Issue : 3

Online ISSN : 2394-5478

Print ISSN : 2394-546X

Article First Page : 154

Article End Page : 158


Abstract

Background and Objectives: Pseudomonas aeruginosa is a common nosocomial pathogen. It is a major cause of multidrug resistance infections in hospitalized patients. Antibiotic resistance increases the morbidity and mortality associated with pseudomonal infections. This leads to rising costs of care resulting from prolonged hospital stay and the need for more expensive drugs. It therefore becomes imperative to study the antibiotic resistance pattern of P.aeruginosa. Objectives of this study were,

1. To isolate and identify Pseudomonas aeruginosa from various clinical samples.
2. To determine the antibiotic susceptibility pattern of these isolates.
3. To detect MBL and Amp C production in the isolates.
Methods: 250 isolates of P.aeruginosa obtained from various clinical samples were identified using standard procedures. The antibiotic susceptibility patterns of the isolates were determined by disc diffusion method for the routinely used antibiotics as per the CLSI guidelines, Minimum inhibitory concentrations (MIC) of the isolates to meropenem was determined using micro broth dilution and agar dilution method. The isolates were also tested for the presence of AmpC (Disc antagonism) and MBL (Imipenem (IMP)-EDTA Combined disk test).
Results: Of the 250 P.aeruginosa included in the study, 238 were isolated from Pus/swab and 12 from urine samples. By disc diffusion method third generation cephalosporin resistance and carbapenem resistance was observed at 30.7% and 28.6% respectively in pus/swab isolates and 50% and 24% respectively among urine isolates. The MIC values of the tested isolates ranged from <0.5 to 16 μg/mL with a MIC50 and MIC90 values of 2 μg/mL and 16 μg/mL respectively. 12.8% of the strains produced AmpC and 18% produced MBL. One isolate was observed to produce both AmpC and MBL.
 
Keywords: Pseudomonas aeruginosa, Meropenem, AmpC, MBL