International Journal of Pharmaceutical Chemistry and Analysis


Evaluation of Anti-Cancer Activity of Newly Synthesized Cobalt Complex of Coumarin Schiff Bases


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Author Details: Ashwini Mallikarjun Rayaji, AHM Vishwanathaswamy Agadihiremath

Volume : 3

Issue : 1

Online ISSN : 2394-2797

Print ISSN : 2394-2789

Article First Page : 1

Article End Page : 12


Abstract

Background: The present study was designed to evaluate the newly synthesized cobalt complex of coumarin schiff base of vanillin (CCV) against diethylnitrosamine (DEN) induced and phenobarbital promoted hepatocellular carcinoma in rats.
Material and Methods: Hepatocelluar carcinoma was induced by DEN at a single dose of 200 mg/kg intraperitoneally followed by administration of phenobarbital (0.05% w/v) daily from 2nd week through drinking water up to 16 successive weeks and synthesized CCV given orally at a dose of 10 mg/kg five days a week for the next 16 weeks. Cancer biomarker, oxidative stress and histopathological studies were carried out to assess the chemopreventive property of the compound.
Results: vanillin Coumarin-cobalt complex at a dose of 10 mg/kg decreased the elevated level of alpha fetoprotein, biochemical markers and increases the reduced level of antioxidant enzymes (p<0.01).
Discussion: The carcinogenic potential of DEN generates reactive oxygen species (ROS), which modify cellular functions such as signal transduction pathways and expression of genes related to generation of mitogenic signals. These pathways lead to conversion of normal cell into cancer cell. The antitumor activity of coumarin is believed to be its metabolic product 7-hydroxycoumarin. The studies showed that there is significant elevation of liver biochemical parameters in DEN+PB treated group. The vanillin coumarin-cobalt complex treated animals showed decreased the elevated levels of biochemical parameters and increase the decreased levels of antioxidant enzymes.
Conclusion: The present findings suggest that chemopreventive property of vanillin coumarin-cobalt complex could be due to decreased levels of cancer marker alpha fetoprotein and DNA content as well as scavenging of free radicals. Histopathological reports further confirms chemopreventive property.

Key words:
Alfa – fetoprotein, Coumarin, Hepatocelluler carcinoma, Vanillin – cobalt complex