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International Journal of Pharmaceutical Chemistry and Analysis


Quantification of Spironolactone by first and second order UV Derivative Spectrophotometry in bulk and tablet dosage form


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Author Details: Parixit Prajapati, Chandni Chandarana, Vishalkumar Modi, Tarashankar Basuri

Volume : 3

Issue : 3

Online ISSN : 2394-2797

Print ISSN : 2394-2789

Article First Page : 168

Article End Page : 173


Abstract

For determination of Spironolactone in pharmaceutical formulation, Simple, fast and reliable first and second order derivative UV spectrophotometric methods were developed. Spectrophotometrically, Spironolactone was determined by measuring the 1D and 2D-values at 226 nm and 262 nm using methanol as background solvent. The Calibration curves were shown linear within a concentration range from 5-35 μg/ml. The developed and validated method was easily applied to the analysis of the pharmaceutical tablet preparations. The percentage recoveries were found to be 100% for given methods. The excipients did not interfere in the analysis. The results showed that this method can be used for rapid determination of Spironolactone in pharmaceutical tablet with specificity and accuracy.

Keywords:
Zero crossing, First order Derivative, Second order Derivative UV spectrophotometry, Spironolactone, Validation