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International Journal of Pharmaceutical Chemistry and Analysis


Simultaneous Determination of Dexibuprofen and Tramadol HCl by HPTLC Method


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Author Details: Prachi Pimple

Volume : 4

Issue : 3

Online ISSN : 2394-2797

Print ISSN : 2394-2789

Article First Page : 79

Article End Page : 82


Abstract

Introduction: Aim of the study is to develop simple, specific, accurate HPTLC method for simultaneous determination of the Dexibuprofen and Tramadol HCl in pharmaceutical dosage form.
Materials and Method: Stationary phase was precoated silica gel 60 F254. The Mobile Phase used was mixture of Chloroform: toluene: ethanol: glacial acetic acid (8:4:1:0.5 v/v/v/v). The detection of the spots was carried out at 264nm and 271nm for Dexibuprofen and Tramadol HCl respectively.
Result: The method was validated in terms of linearity, accuracy, precision and specificity. The Limit of detection for Dexibuprofen and Tramadol HCl was found to be 1µg and 0. 2 µg respectively. The Limit of quantification for Dexibuprofen and Tramadol HCl was found to be 1.6µg and 0.4µg respectively.
Conclusion: The method is free from the interferences due to excipients present in the formulation and can be successfully used for determine drug in pharmaceutical dosage form.

Keywords:
Dexibuprofen, Tramadol HCl, HPTLC method, Simultaneous determination

Doi No:-10.18231