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International Journal of Pharmaceutical Chemistry and Analysis


DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR SIMULTANEOUS ESTIMATION OF CILNIDIPINE AND VALSARTAN IN BULK AND TABLET DOSAGE FORM


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Author Details: Ritesh P. Bhole.Vinayak C. Pawara, Sohan S. Chitlange, Sager B. Wankhede

Volume : 2

Issue : 2

Online ISSN : 2394-2797

Print ISSN : 2394-2789

Article First Page : 102

Article End Page : 107


Abstract

Cilnidipine and Valsartan in combined dosage form has been developed and validated. Sample and standard solutions of Cilnidipine and Valsartan were applied to precoated silica gel G 60 F254 HPTLC plates and the plates were developed with Toluene: Methanol: Ethyl acetate: Glacial Acetic acid in the ratio 8:1:1:0.1 (v/v/v/v) as mobile phase. The Rf value for cilnidipine and Valsartan was found to be 0.29 min and 0.56 min respectively. The detection was performed at 240 nm. The calibration curve was found to be linear between 1000 to 6000 ng/band for cilnidipine and 8-48 μg/band for Valsartan with correlation coefficients 0.993 and 0.993 for Cilnidipine and Valsartan respectively. Accuracy and precision of the proposed method was evaluated by recovery studies (% recovery for Valsartan and Cilnidipine was 99.03 % and 99.86 %).The results have been validated statistically as per ICH guidelines.


Keywords: Validation, Valsartan, Cilnidipine, HPTLC

Doi No:-10.18231