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Indian Journal of Pathology and Oncology


Role of oxidative stress and antioxidant levels in tubercular, reactive and metastatic lymphadenopathy - A comparative study


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Author Details: Parineeta B. Magdum, Manisha A. Atram, Nitin Gangane

Volume : 4

Issue : 4

Online ISSN : 2394-6792

Print ISSN : 2394-6784

Article First Page : 540

Article End Page : 545


Abstract

 Objectives:
1.        To evaluate the serum levels of lipid peroxidation product like thiobarbituric acid reacting substance(TBARS) i.e. Malondialdehyde and Reactive oxygen species(ROS) i.e. Nitric oxide in patients of tubercular, reactive and metastatic lymphadenopathy.
2.        To evaluate the antioxidants status i.e. level of ascorbic acid, reduce glutathione and superoxide dismutase in patients of tubercular, reactive and metastatic lymphadenopathy.
Materials and Method: Forty cases each of tubercular lymphadenitis, reactive lymphadenitis and metastatic lymphadenopathy was diagnosed on FNAC and evaluated for various parameter i.e. Malondialdehyde, Nitric oxide, ascorbic acid, reduced glutathione and superoxide dismutase by colorimetric method. Thirty healthy individuals were taken as controls.
Results: Levels of malondialdehyde and nitric oxide were significantly raised in patients of tubercular lymphadenitis (6.89 ± 1.48nmol/ml) and metastatic lymphadenopathy (8.03±1.51nmol/ml) as compare to patients with reactive lymphadenopathy (4.62 ± 1.14nmol/ml) and control group (4.62 ± 1.45nmol/ml). Superoxide dismutase, reduced glutathione and Ascorbic acid reduce in tubercular (2.14 ± 0.63units/ml) as well as metastastic lymphadenopathy (1.88 ± 0.52units/ml) than reactive lymphadenopathy (3.19 ± 0.54units/ml) and control group (3.42 ± 0.53units/ml).
Conclusion: ROS are known to play tremendous role in health and disease. A frequent cellular target to ROS is lipid components of the cell membrane resulting in lipid peroxidation. To counter the harmful effects of ROS, antioxidants defense mechanism operates to detoxify or scavenge these ROS. This may be important for better understanding the pathogenesis of the disease and may contribute to its diagnosis and treatment.

Keywords:
Oxidative stress, Antioxidants, Lymphadenopathy, Tuberculosis, Metastatic

Doi No:-10.18231