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Indian Journal of Pharmacy and Pharmacology


Chicken ileum: a better option for conducting isolated tissue experiments and bioassay


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Author Details: VM Motghare, MB Nandeshwar, CS Bajait, SA Pimpalkhute, SD Sontakke

Volume : 4

Issue : 2

Online ISSN : 2393-9087

Print ISSN : 2393-9079

Article First Page : 110

Article End Page : 113


Abstract

In vivo method of animal testing is the use of non-human animals in experiments. Mice, rats, rabbits, guinea pigs, hamsters and non-human primates are widely used as laboratory animals. In vitro cell culture technique and in silico computer simulation are alternatives to in vivo animal testing. Current limitations on performing experiments with laboratory animals bring about the need for search of alternate tissues for biological testing. Bioassay is an integral part of undergraduate and postgraduate Pharmacology curriculum where various isolated tissues like frog rectus, rat colon, guinea pig ileum etc. were used for which whole animal has to be sacrificed for a small piece of tissue. So, we conducted bioassay of acetylcholine on chicken ileum obtained from chicken sacrificed for food which is easily available from slaughter houses. We found greater height of response and stability with Tyrode solution compared with Ringer Locke solution. We assessed the unknown concentration of acetylcholine by interpolation method of bioassay which we found 82.17% correct of actual concentration of unknown. Isolated chicken ileum is a better option available for conducting isolated tissue experiments considering the restriction with the use of experimental animals under CPCSEA guidelines.

Keywords
: Chicken ileum, Bioassay, Acetylcholine, Physiological salt solution, Interpolation