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The Journal of Community Health Management


CLINICO-BIOCHEMIAL STUDY OF OXIDATIVE STRESS IN COPD PATIENTS IN A TERTIARY CARE HOSPITAL


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Author Details: J K Mishra, Ashvendra Singh Chawra, Girish Singh, H D Khanna

Volume : 2

Issue : 2

Online ISSN : 2394-2738

Print ISSN : 2394-272X

Article First Page : 58

Article End Page : 63


Abstract

Background: Chronic obstructive pulmonary disease (COPD) characterized by airflow limitation that is usually progressive and associated with an enhanced chronic inflammatory response in the airways is a major worldwide health problem that has an increasing prevalence and mortality.
Aim: The aim of study was to estimate serum malondialdehyde (MDA) and Antioxidant level from blood samples of COPD patients and to compare with age and sex matched healthy controls and further to compare these levels between severity grades and smoking pack years.
Methods: The present study was conducted in the Department of TB and Respiratory disease, Institute of Medical Sciences, Banaras Hindu University, Varanasi during June 2012 to June 2013. A total of 44 COPD patients who met the prescribed inclusion and exclusion criteria were registered for the study and 44 age and sex matched healthy non COPD individuals were selected as the controls. MDA level was estimated by thiobarbiburic acid assay by Philpot Method and assay of TNF-α done by TNF-α (Human) ELISA KIT Protocol.
Results: The percentage of male cases was 72.7 and 47.1% of cases were over 60 years of age. 50% cases belonged to poor socioeconomic status. Cough, sputum production and dyspnoea were the predominant symptoms. 40.9% cases had history of previous hospitalization. A statistically significant difference in mean MDA as well as total antioxidant level was found between cases and controls. It was observed that the level of MDA increases and total antioxidant level decreases as severity of COPD increases. The level of MDA increased and level of total antioxidant decreased with increase in the smoking pack years. Conclusion The inflammatory mediators involved in COPD have been clearly defined, and an early approach on smoking cessation and controlling oxidative stress may retard disease progression.

Key words: Malondialdehyde, MDA, Antioxidant