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Journal of Oral Medicine, Oral Surgery, Oral Pathology and Oral Radiology


A comparative evaluation of whole blood total antioxidant capacity using nitroblue tetrazolium reduction test in patients with oral lichen planus and healthy subjects


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Author Details: Pallavi P. Channe, Richa Kelwalramani, Shraddha P Channe, Angha V. Shete, Ladusingh Rajpurohit

Volume : 2

Issue : 2

Online ISSN : 2395-6194

Print ISSN : 2395-6186

Article First Page : 58

Article End Page : 61


Abstract

Background and Objectives: Oral Lichen Planus is an inflammatory disease of unknown etiology. Recently, reactive oxygen species have been implicated in the pathogenesis of this disease. The investigation of disease associated oxidant-antioxidant imbalance is hard because constrained availability of specific biomarkers of oxidative stress, and the fact that measurement of individual antioxidant may give a deceptive image due to the fact that antioxidants work in concert through chain breaking reactions. Therefore, analysis of total antioxidant capacity may be the maximum applicable investigation. As blood is continuously exposed to oxidative stress, the aim of this study was to analyze overall blood antioxidant potential in healthy and oral lichen planus patients through the use of Nitroblue Tetrazolium reduction test.
Methods: The study included 30 individuals which were selected from outpatients department with age ranging between 30 and 60 years, 15 patients in oral lichen planus group and 15 in healthy group (control), respectively. Blood samples were collected early morning and tested using Nitroblue Tetrazolium Reduction test (NBT). The test was performed and gave us the clear picture of whole blood total antioxidant capacity.
Results: Results were obtained,  analyzed and results showed that the total antioxidant capacity in whole blood of patients with oral lichen planus was significantly decreased  as compared with healthy group (P<0.001).
Interpretation and conclusion: The decreased antioxidant capacity of total blood in oral lichen planus is indicative of the important role played by oxidative stress within the etiopathogenesis of oral lichen planus and warrants in addition investigation as it may offer a connection between chronic inflammatory diseases like oral lichen planus and plenty of other sicknesses that may be related to free radical formation.

Keywords:
Lichen Planus, Oxidative Stress, Antioxidants, Reactive oxygen species (ROS)