Journal of Pharmaceutical and Biological Sciences


Screening of IGF-I and IGFBP-3 genes polymorphism in popular Goat breeds in Egypt


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Author Details: Mohamed Abdel Hafez, Karima Mahrous, Sekena H. Abdel-Aziem, Mohamed Abdel Mordy

Volume : 5

Issue : 1

Online ISSN : 2320-1924

Print ISSN :

Article First Page : 8

Article End Page : 14


Abstract

The genetic polymorphisms of two functional genes; Insulin-like growth-I (IGF-I) and Insulin-like growth factor binding protein-3 (IGFBP-3) were investigated in three goat breeds (Barki, Damascus and Zaraibi) by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP), for differentiating these breeds. IGF-I-HaeIII/RFLP revealed three genotypes (CC, CG and GG) with frequencies; 0.40, 0.10 and 0.50, respectively in Barki. Moreover, the expected heterozygosity (He) value (0.51) was higher than observed heterozygosity (Ho) value (0.10) in Barki. Chi-square (χ2) value in Barki (13.6) showed a significant deviation from Hardy-Weinberg equilibrium [HWE] (P<0.05). IGFBP-3-XspI/RFLP revealed three genotypes (CC, CT and TT), with only CT and TT in all breeds. CT frequency was the highest in Barki (0.80), and TT frequency was the highest in Damascus (0.75). Ho value was higher than He value in all breeds. Additionally, Ho, He and χ2 values were the highest in Barki (0.80, 0.49 and 8.26, respectively) and χ2 value showed a significant deviation from HWE (P<0.05). The polymorphisms demonstrated in these results are recommended as effective markers for genetic differentiation among goat breeds, opening up interesting prospects for goat breeders for future selection programs.

Keywords: Genetic Polymorphism, goat, PCR-RFLP, IGF-I gene, IGFBP-3 gene