Journal of Pharmaceutical and Biological Sciences


Comparative analysis of efficiency of pore size and materials of filters for the isolation and purification of leptospires


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Author Details: Prabhusaran Nagarajan, Natarajaseenivasan Kalimuthusamy, Joseph Pushpa Innocent Danialas

Volume : 5

Issue : 5

Online ISSN : 2320-1924

Print ISSN :

Article First Page : 199

Article End Page : 203


Abstract

Filtration is a major procedure performing in the culture media preparation and avoiding contamination during sub culturing of leptospiral strains. The main objective of this study is to determine the effective filter material and pore size by compare among various filters. The filter materials evaluated in this study are glass fiber (1μm pore diameter), millipore polyvinylidene fluoride (0.22μm and 0.45μm) nitrocellulose (0.22 and 0.45μm pore diameters) and nylon mesh (37μm). The leptospiral cultures in aqueous was filtered by using all filters for the determination of effective filtration of leptospires. All the filtrates were examined by dark field microscopy and the effective filters were selected for further analysis. The filtrate also inoculated in bacterial and fungal culture media to find out any contamination found. Our results suggested that the isolation of 100% leptospires using millipore polyvinylidene fluoride and the optimal pore diameter should be 0.45μm from EMJH broth with well cultured leptospires. Although filtration method can be applicable for isolation and purification of leptospires, it is unclear whether this is a useful method in all situations. This study concluded with the statement “large proportion of leptospires can be filtered by membrane filter at the pore size of 0.45μm”.

Keywords:
Leptospira, filtration technique, pore size, EMJH broth